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. 2012 Oct 8;7(10):e46208. doi: 10.1371/journal.pone.0046208

Figure 2. Khz induces apoptosis in transformed cells.

Figure 2

(A) (A-a) The shape and type of fused fruiting bodies. (A-b) Hyphae isolated from a Ganoderma lucidum mushroom on a Petri dish. (A-c) Shape of G. lucidum. (A-d) Shape and type of fused fruiting bodies and hyphae from Polyporus umbellatus. (A-e) Fusion of G. lucidum and P. umbellatus. (A-f) The fused hyphae of G. lucidum and P. umbellatus. (A-g) Agar-cultured fusion fungi. (A-h) DNA from fused hyphae (Khz). (A-i) Cultivation conditions for Khz. (B) Analysis of apoptosis using annexin-V-FITC (An) and propidium iodide (PI) staining. HepG2 cells were treated with a 1∶2 dilution of Khz, and apoptosis was analyzed after 0.5, 1, and 2 h by flow cytometry. Data are representative of more than 3 experiments. (C) HepG2 cells were treated with a 1∶2 dilution of Khz and stained with An and PI for flow cytometric analysis. Data represent mean ± SD. (D) BEAS-2B, 1799, 1198, and 1170-I cells were treated with Khz (1∶2 dilution), and apoptosis was examined by PI staining followed by flow cytometric analysis. Data are representative of more than 3 experiments.