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. 2012 Oct 8;7(10):e47049. doi: 10.1371/journal.pone.0047049

Figure 5. AA005 induces autophagy of colon cancer cells.

Figure 5

(A) Immunoblot analysis of LC3-I and LC3-II levels in LOVO cells treated with AA005. (B) LOVO cells transfected with pQCXIP-GFP or pQCXIP-GFP-LC3 plasmid were treated for 24 h with AA005 (100 nM) and/or inosine (20 mM), and rapamycin (100 nM), and assessed by immunofluorescence analyses. (C) LOVO cells were treated with indicated protocols, lysed, and Western blot assay was performed using indicated antibodies. (D) LOVO cells were treated with AA005 or cisplatin (20 µM) for 24 h, or AA005 at 100 nM for indicated time points. The cells were then analyzed by Annexin V/PI staining and flow cytometry. (E) Western blot analyses of lysates of LOVO cells treated with AA005 or cisplatin (20 µM for 24 h).