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. 2012 Oct 8;7(10):e44686. doi: 10.1371/journal.pone.0044686

Figure 1. Development and optimization of an ARE induction assay in AREc32 Cells.

Figure 1

(A) The luciferase assay signal stability in the presence of 0, 10, 20, or 80 µM tBHQ.(B) The effect of AREc32 cells seeding density on the luciferase assay sensitivity in the presence of 0, 10, 20, or 80 µM. (C) The effect of DMSO concentration on the luciferase assay sensitivity using a range of 0%–10% DMSO in the presence or absence of 0 or 10 µM tBHQ.