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. 2012 Oct 8;7(10):e44686. doi: 10.1371/journal.pone.0044686

Figure 2. Validation of the ARE induction assay using known Nrf2 activators and through pilot screening.

Figure 2

(A) Concentration-response curves of tBHQ and CDDO-Im to increase the luminescent signal in AREc32 cells. (B) Activity spread of compounds in Microsource, Prestwick, and CMLD libraries together with positive controls. Compounds in Microsource and CMLD libraries were tested at 10 µM, and compounds in Prestwick library were tested at 8 µM. (C) Z′ scores for 16 pilot screening plates.