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. 2012 Oct 8;7(10):e46112. doi: 10.1371/journal.pone.0046112

Figure 9. Brefeldin A sensitivity of Tex1 export.

Figure 9

3D7 infected RBC were treated with BFA and fixed (+BFA). Tex1 was stained using P27 or P27A-specific mouse antibodies (in red, upper panel: early trophozoite, middle panel: trophozoite). Tex1 visible inside the parasite in close proximity to the nucleus. A control culture (+ETOH) was incubated with equivalent concentration of ethanol, the solvent of Brefeldin A. In the control culture Tex1 was correctly exported and associated to MC (in red). The nucleus was stained with DAPI (in blue). Transmission image (DIC). scale bar: 5 µm.