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. 2012 Oct 8;7(10):e47389. doi: 10.1371/journal.pone.0047389

Figure 3. Growth indices of PIGA-targeted and non-targeted cell populations.

Figure 3

Cells derived from HCT116 (left) and DLD-1 (right) were propagated in 96-well tissue culture plates, and cell numbers assayed at Day 3, Day 5, and Day 7 were shown relative to those at Day 1. In each cluster of bar graphs, open bars represent a FLAER-negative bulk cell population and PIGA-targeted (confirmed by Southern blotting) single cell clones #1, #2 and #3 (from left to right); gray bars represent a FLAER-positive bulk cell population and PIGA-non-targeted (determined by analytical PCR) single cell clones #1, #2 and #3 (from left to right); a filled bar represents the parental cell line (mean ± s.d.; n = 6).