Figure 1. Specific down-regulation of Mybbp1a in wild type ES cells blocks proliferation and induces activation of caspase-3.

(A) ES cells were infected with the Mybbp1a-specific shRNA3 or with an empty non-target (NT) lentivirus vector (see Materials and Methods), and the proliferation rate was measured starting two days after infection (0 h) every 24 h, using a cell counter. (B). At the end of the experiment (72 h) the cells were lysed and the extract immunoblotted with Mybbp1a-specific antibodies (anti-p160C) using Tubulin as loading control. (C, D). The extracts were also immunoblotted against Oct4 (for specificity of the down-regulation) and cleaved-Caspase 3 for apoptosis using Vinculin as loading control.