Table 4. Validation by Q-PCR of the gene expression profile in MYBBP1A-depleted HeLa cells. #.
Gene | Ratio* |
CKS1 | 0.977+/−0,085632938*** |
GADD45B | 4.88+/−0,886155931*** |
CDKN1A (P21) | 10.45+/−0,449326533*** |
SFN | 1.29+/−0,088121129 |
NFKB2 | 2.9+/−0,0675** |
cJUN | 3.05+/−0,246*** |
MYBBP1A | 0.09+/−0,00665*** |
TOP2A | 0.27+/−0,014977761*** |
TOP2B | 0.21+/−0,030892286*** |
WEE1 | 0.81+/−0,144244006** |
BRCA1 | 0.35+/−0,029816103*** |
CDK7 | 0.76+/−0,097858742** |
Real-Time PCR was performed on HeLa control (High-GC) or MYBBP1A-depleted HeLa cells (siRNA1) with the same RNA samples used for microarray experiment. Two biological replicates were used for each condition and the experiment was performed in triplicate; an arbitrary value of 1 was given to the control (CTL).
Ratio between the expression in down-regulated vs. control (see Methods) cells.
p≤0.01.
p≤0.001 (t test).