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. 2012 Oct 8;7(10):e39723. doi: 10.1371/journal.pone.0039723

Table 4. Validation by Q-PCR of the gene expression profile in MYBBP1A-depleted HeLa cells. #.

Gene Ratio*
CKS1 0.977+/−0,085632938***
GADD45B 4.88+/−0,886155931***
CDKN1A (P21) 10.45+/−0,449326533***
SFN 1.29+/−0,088121129
NFKB2 2.9+/−0,0675**
cJUN 3.05+/−0,246***
MYBBP1A 0.09+/−0,00665***
TOP2A 0.27+/−0,014977761***
TOP2B 0.21+/−0,030892286***
WEE1 0.81+/−0,144244006**
BRCA1 0.35+/−0,029816103***
CDK7 0.76+/−0,097858742**
#

Real-Time PCR was performed on HeLa control (High-GC) or MYBBP1A-depleted HeLa cells (siRNA1) with the same RNA samples used for microarray experiment. Two biological replicates were used for each condition and the experiment was performed in triplicate; an arbitrary value of 1 was given to the control (CTL).

*

Ratio between the expression in down-regulated vs. control (see Methods) cells.

**

p≤0.01.

***

p≤0.001 (t test).