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. 2012 Oct 8;7(10):e46731. doi: 10.1371/journal.pone.0046731

Figure 6. The localization of TGF-β1 and -β2 mRNA in relation to markers of the penumbra and glial scar.

Figure 6

A: Double labeling of TGF-β1 mRNA (black in situ hybridization signal) and immunoreactivity of heat shock protein 70 (Hsp70), a marker of the penumbra (brown precipitate) 24 h following MCAO. The lesion is indicated by star symbols (*), and the lesion border is demarcated by black dots. Black squares demarcate the outer border of the penumbra. The framed area in Aa is shown in Ab to better appreciate the similar distribution of TGF-β1 mRNA and Hsp70 in the penumbra. Ac is a high magnification image of the framed area in Ab and demonstrates that TGF-β1 mRNA and Hsp70 immunoreactivity are not co-localized in the same cells. B: Double labeling of TGF-β2 mRNA (black in situ hybridization signal) and Hsp70 immunoreactivity (brown precipitate) 24 h following MCAO. Black squares demarcate the outer border of the penumbra. Within the penumbra, the majority of the TGF-β2 mRNA-expressing cells contain Hsp70 immunoreactivity. However, TGF-β2 mRNA expressing cells but not Hsp70-immunoreactive cells are present in the intact brain tissue. C: A glial scar can be identified based on the high density of intensely labeled GFAP-positive astrocytes. TGF-β1-expressing cells are present within the glial scar and also within the lesion but not in the intact tissue outside the glial scar. Abbreviations: LV - lateral ventricle. Scale bars = 1 mm for Aa, 400 µm for Ab, 100 µm for Ac, and 200 µm for both B and C.