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. 2012 Oct 8;7(10):e46731. doi: 10.1371/journal.pone.0046731

Table 1. Immunolabeling of TGF-β-expressing cells.

TGF-β1 TGF-β2 TGF-β3
layer II layer V CP layer II layer V layer II
Number of TGF-β cells/0.08 mm2 18.3±4.9 16.7±3.5 20.5±4.5 18.3±4.2 17.0±3.9 14.0±4.5
Immunolabeled cells in the % of TGF-β cells
NeuN 3.7±2.8 4.7±3.3 5.7±3.9 87,1±3,8 84,8±2,7 81,1±3.5
GFAP 26.3±2.8 21.6±6.2 22.2±4.4 4.2±2.3 4.0±2.1 9.3±5.8
Iba1 93.7±3.4 94.0±4.6 96.0±4.1 3.1±2.6 3.5±2.4 6.7±3.4
Hsp70 7.2±2.2 4.8±3.9 5.5±2.9 76.3±7.4 69.2±8.9
Fos 4.1±2.2 4.7±3.3 5.2±2.7 90.2±3.8 91.9±4.6
ATF-3 8.4±1.9 7.1±2.6 8.5±4.1 2.8±1.9 4.3±2.9

Data on TGF-β1 is presented in 3 different locations, layer II and V of the cerebral cortex and the caudate putamen (CP). Because TGF-β2 is not expressed in the caudate putamen, TGF-β2-expressing cells were only counted in the cerebral cortex. Similarly, data on TGF-β3 are provided only for layer II of the cerebral cortex. In the upper row, the total number of TGF-β-expressing cells counted in a 400×200 µm rectangular-shaped area immediately outside the lesion is shown. The number of double-labeled cells was also counted in the same field, and the calculated ratios were averaged. One-way ANOVA did not indicate significant differences between brain regions for any of the markers.