Table 1. Immunolabeling of TGF-β-expressing cells.
TGF-β1 | TGF-β2 | TGF-β3 | ||||
layer II | layer V | CP | layer II | layer V | layer II | |
Number of TGF-β cells/0.08 mm2 | 18.3±4.9 | 16.7±3.5 | 20.5±4.5 | 18.3±4.2 | 17.0±3.9 | 14.0±4.5 |
Immunolabeled cells in the % of TGF-β cells | ||||||
NeuN | 3.7±2.8 | 4.7±3.3 | 5.7±3.9 | 87,1±3,8 | 84,8±2,7 | 81,1±3.5 |
GFAP | 26.3±2.8 | 21.6±6.2 | 22.2±4.4 | 4.2±2.3 | 4.0±2.1 | 9.3±5.8 |
Iba1 | 93.7±3.4 | 94.0±4.6 | 96.0±4.1 | 3.1±2.6 | 3.5±2.4 | 6.7±3.4 |
Hsp70 | 7.2±2.2 | 4.8±3.9 | 5.5±2.9 | 76.3±7.4 | 69.2±8.9 | – |
Fos | 4.1±2.2 | 4.7±3.3 | 5.2±2.7 | 90.2±3.8 | 91.9±4.6 | – |
ATF-3 | 8.4±1.9 | 7.1±2.6 | 8.5±4.1 | 2.8±1.9 | 4.3±2.9 | – |
Data on TGF-β1 is presented in 3 different locations, layer II and V of the cerebral cortex and the caudate putamen (CP). Because TGF-β2 is not expressed in the caudate putamen, TGF-β2-expressing cells were only counted in the cerebral cortex. Similarly, data on TGF-β3 are provided only for layer II of the cerebral cortex. In the upper row, the total number of TGF-β-expressing cells counted in a 400×200 µm rectangular-shaped area immediately outside the lesion is shown. The number of double-labeled cells was also counted in the same field, and the calculated ratios were averaged. One-way ANOVA did not indicate significant differences between brain regions for any of the markers.