Figure 6. Equilibrium renaturation plots of GFPnt and GFPhs-r5M variants (fraction of recovered fluorescence).
Urea-denatured protein samples were diluted in different concentrations of urea in refolding buffer and recovered fluorescence was normalized by dividing it by fluorescence of corresponding non-denatured samples diluted in similar fashion. Inlet table shows the aconcentration of urea at which the 50% of fluorescence is recovered during renaturation process under urea-unfolded conditions.