Table 1.

Nitric oxide formation by T. cruzi epimastigotes

Compound (mM)	NO, nM⋅min−1⋅mg−1	% change
In Krebs–Henseleit buffer
None	1.94  ± 0.19a	0
l-Arginine (1)	2.29  ± 0.28b	18
l-NAME (4)	1.55  ± 0.18b	−20
MK-801 (1)	1.96  ± 0.33	1
l-Arginine (1) + l-Glutamate (1)	3.10  ± 0.05b	60
l-Arginine (1) + l-Glutamate (1) + l-NAME (4)	1.84  ± 0.14	−5
l-Arginine (1) + l-Glutamate (1) + MK-801 (0.1)	1.87  ± 0.1	−3
d-Arginine (1) + l-Glutamate (1)	1.69  ± 0.14	−13
In the presence of 10% FHS
10% heat-inactivated human serum	2.32  ± 0.23	0
10% FHS	2.36  ± 0.18c	2
+ l-Glutamate (1)	2.41  ± 0.13	4
+ l-Arginine (10)	3.98  ± 0.37d	72
+ l-Arginine (10) + l-Glutamate (1)	3.92  ± 0.3d	69
+ l-Arginine + l-NAME (10)	2.10  ± 0.12	−10
+ l-Arginine (10) + MK-801 (0.1)	1.94  ± 0.26d	−16

^⋅NO production was measured as NO accumulation over a 10-h period. Results are expressed as mean ± SD of at least three independent experiments.  

^b indicates statistical difference (P < 0.05) with respect to  

^a;  

^d indicates statistical difference (P < 0.05) with respect to  

^c.