Table 2.
[3H]Thymidine incorporation and DNA fragmentation
| Compound (mM) | [3H]Thymidine incorporation, % | DNA fragmentation, % |
|---|---|---|
| None | 100 ± 10 | 15 ± 4 |
| 10% FHS | 47 ± 9a | 100 ± 20c |
| + l-Arginine (1) | 57 ± 8 | 99 ± 10 |
| + l-Arginine (5) | 70 ± 4b | 20 ± 4d |
| + l-Arginine (10) | 84 ± 6b | 18 ± 9d |
| + l-Arginine (10) + l-NAME (10) | 45 ± 7 | 115 ± 23 |
| + d-Arginine (10) | 50 ± 10 | 100 ± 14 |
| + Agmatine (10) | 100 ± 17b | 20 ± 3d |
| + Agmatine (10) + l-NAME (10) | 82 ± 3b | 17 ± 2d |
| + Putrescine (10) | 93 ± 8b | 16 ± 5d |
| + Putrescine (10) + l-NAME (10) | 82 ± 3b | 18 ± 6d |
| + Spermidine (10) | 88 ± 6b | 15 ± 10d |
| + Spermidine (10) + l-NAME (10) | 100 ± 10b | 17 ± 7d |
| + DFMA (10) | 60 ± 8 | 110 ± 10 |
| + DFMA (10) + l-Arginine (10) | 58 ± 7 | 15 ± 2d |
| + DFMA (10) + l-Arginine (10) + | ||
| l-NAME (10) | 60 ± 5 | 120 ± 15 |
[3H]Thymidine incorporation is expressed relative to nontreated epimastigotes (control condition) taken as 100%. Percent DNA fragmentation was estimated by digital analysis of the electrophoretic bands, taking as 100% the fragmentation observed with 10% FHS. Results are mean ± SD of at least three independent experiments.
b
and
d
indicate statistical significance (P < 0.05) compared with
a
and
c
, respectively.