Figure 6. Effects of Ctsg-PML-RARA on flow-sorted promyelocytes.

A. Experimental schema. Bone marrow cells from Ctsg-PML-RARA and littermate WT controls were harvested and KLS and promyelocytes were purified by flow sorting. Aliquots from individual donor mice were plated in methylcellulose containing myeloid cytokines (SCF, IL-3, IL-6, Epo). KLS cells were combined from all donors by genotype and 3,000 cells/recipient were transferred to sub-lethally irradiated Ly5.1 mice (4 recipients per genotype). Purified promyelocytes were separated into two pools, and 50,000 cells/recipient were transferred into sub-lethally irradiated Ly5.1 mice. B. Total CFUs per 10,000 KLS cells plated by week. Every 7 days, total colonies were counted. Total cells were then collected in warm (37°) media and serially replated. C. Total CFUs per 10,000 promyelocytes plated by week. D. Percentage of donor CD45.2⁺ cells in total bone marrow cells of individual recipient mice 12 weeks after engraftment, with indicated flow sorted cells. All mice that received promyelocytes had <1% CD45.2⁺ cells. E. Percentage of CD45.2⁺ cells within the Gr1⁺, CD3⁺, and CD19⁺ bone marrow cells of mice engrafted with promyelocytes.