Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Nov 15.

Published in final edited form as: J Neuroimmunol. 2012 Aug 9;252(1-2):40–48. doi: 10.1016/j.jneuroim.2012.07.009

Fig. 4 — Tubby facilitates primary microglial phagocytosis. (A) Primary microglial phagocytosis. pHrodo-labeled membrane vesicles were preincubated with GST-tubby or GST, washed, incubated with primary neonatal microglia for phagocytosis in the presence or absence of excess MerFc and analyzed by confocal microscopy as in Fig. 3. Bar = 20 µm. (B) Percentage of microglia with internalized pHrodo signal were quantified (± SEM, n>100; t-test).

Fig. 4 — Tubby facilitates primary microglial phagocytosis. (A) Primary microglial phagocytosis. pHrodo-labeled membrane vesicles were preincubated with GST-tubby or GST, washed, incubated with primary neonatal microglia for phagocytosis in the presence or absence of excess MerFc and analyzed by confocal microscopy as in Fig. 3. Bar = 20 µm. (B) Percentage of microglia with internalized pHrodo signal were quantified (± SEM, n>100; t-test).