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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: J Immunol. 2012 Sep 17;189(8):3815–3821. doi: 10.4049/jimmunol.1201431

Figure 5. KLF2 expression induces CCR5 in some but not all transformed T cell lines and restores susceptibility to R5 infection.

Figure 5

A. KLF2-expressing CEM, Jurkat and SupT1 cells were sorted by GFP expression GFP+ cells on day 0, and then monitored for stability of expression subsequently at day 1 and day 11. B. Relative CCR5 expression levels were measured by quantitative PCR using cDNA obtained from freshly (day 1) sorted KLF2-expressing CEM, Jurkat and SupT1 cells, as well as mCD28-167*-expressing and untransduced controls for each human CD4 T cell line. Relative KLF2 and CCR5 expression levels were normalized to those observed in untransduced cells for each T cell line. C. Untransduced and KLF2-, mCD28-167*-, or CCR5-expressing Jurkat T cells were challenged with cell-free HIV-1BaL. After 48 hours, cells were harvested and TaqMan was performed for HIV-1 gag to assess the extent of infection, using DNA from stably-infected Ach-2 cells as a standard. Samples were run in duplicate, and results are indicative of those obtained in three separate experiments.