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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Cell Signal. 2012 Jul 20;24(12):2291–2296. doi: 10.1016/j.cellsig.2012.07.009

Fig. 5.

Fig. 5

Silibinin has no effect on enforced LRP6 expression. HT1080 cells stably transfected with HA-tagged LRP6 or empty pLNCX2 vector in 6-well plates were treated with silibinin at the indicated concentrations. Twenty-four hours later, the levels of HA-LRP6, Axin2 and cytosolic free β-catenin were examined by Western blotting. Samples were also probed with the anti-actin antibody to verify equal loading.