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ssDNA transformation is less efficient than dsDNA transformation and the Crick strand of DUS12 enhances ssDNA transformation in strain FA1090 (A) and MS11 (B). Strain FA1090 (A) or MS11 (B) were quantitatively transformed with equal moles of DUS0 (black bars) and DUS12 (white bars) Crick ssDNA, Watson ssDNA, and plasmid dsDNA (DS) containing gyrB1. Transformation efficiencies are plotted as resistant CFU/total CFU. *p<0.05. Error bars are SEM.
