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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: J Immunol. 2012 Sep 10;189(8):3983–3994. doi: 10.4049/jimmunol.1200626

FIGURE 1. Recognition of A10L peptides by a vaccinia virus-specific CD4 T cell line.

FIGURE 1

(A) The specificity of the vaccinia-specific TCL was tested using IFNγ-ELISPOT. TCL was stimulated with vaccinia virus-infected CV-1 cell lysate (VV, red bar), non-infected CV-1 cell lysate (NI, green bar), and medium only (MED, blue bar), using irradiated autologous PBMC (irPBMC, left panel) or HLA-DR1 matched B-lymphoblastoid cells (LG2,right panel) as antigen presenting cells (APC). The p-value of responses between VV and NI is indicated. This data represent three independent experiments with 4 replicates each. (B, C) The VV-specific CD4 T cell line was stimulated with overlapping A10L peptides to test peptide immunogenicity, shown as spots per well (SPW) in IFNγ-ELISPOT assay using (B) autologous irPBMC (solid blue circle,) or (C) LG2 (solid green circle) as APCs. Medium (MED) was used as control (clear blue circle in (B) and clear green circle in (D) at the very right x-axis). Dashed line represented the threshold we set up for positive peptides (SPW is more than two fold of that of MED, and p value between that peptide and MED is less than 0.01). The positive peptides are labeled with p-value relative to medium control. (B) and (C) represent three independent experiments with at least two replicates each.