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. 2012 Aug 29;32(35):11930–11941. doi: 10.1523/JNEUROSCI.0178-12.2012

Figure 4.

Figure 4.

PCAF recruits ATF4 to the promoter of the immediate early gene zif268, which downregulates its expression. a, ATF4 functionally interacts with the zif268 promoter. b, ATF4 coimmunoprecipitates (IP) with p300 and PCAF in cortical neurons in vitro. c, In the presence of a PCAF inhibitor, H3-CoA-20-Tat, ATF4 binding to the CRE within the zif268 promoter is decreased (two-tailed t test, t = 14.60, df = 6, p < 0.001). d, ATF4 binding to the zif268 promoter is not affected by inhibition of p300/CBP by C646. e, Relative to nonstimulated control samples, 50 mm KCl-induced depolarization (used to mimic neural activity) led to significantly less ATF4 binding to the zif268 promoter in the presence of H3-CoA-20-Tat (F(3,21) = 4.28, p < 0.05; Tukey's post hoc test, Control KCl− vs H3-CoA-20-Tat KCl+, p < 0.05). f, Activity-dependent ATF4 binding to the zif268 promoter is not affected by inhibition of p300/CBP. *p < 0.05.