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. Author manuscript; available in PMC: 2012 Oct 9.
Published in final edited form as: Eur J Immunol. 2010 May;40(5):1386–1395. doi: 10.1002/eji.200940151

Figure 4.

Figure 4

Affinity dependent IL-10 Treg generation during tolerization. Tg4 mice were treated with 14 i.n. doses of MBP Ac1–9 Ac1–9[4K], [4A] or [4Y] peptides. Spleens were isolated from peptide-treated Tg4 mice 2 h after the last peptide treatment. ICCS for IL-2, IFN-γ, IL-4 and IL-10 was performed after an additional 4-h stimulation with PMA and ionomycin. (A) Cells are gated on V8+ cells from each peptide treatment group and analyzed for IL-2, IFN-γ and IL-10 expression with cytokine gates based on isotype controls. (B) Dot plots show co-secretion of IFN-γ and IL-10 by Vβ8+ T cells from each peptide treatment group. Data are representative of at least three independent experiments.