FIGURE 3.

TAL is a superagonist at TRH-R. (A) IP1 production was determined in HEK 293 cells expressing TRH-R in the presence of increasing doses of TAL or TRH as described in Section “Materials and Methods.” The data represent the mean of duplicate points from five experiments. (B) MeTRH inhibits IP1 formation stimulated by TAL and TRH. IP1 production was determined in the presence of TRH (0.1 μM), TAL (3 μM), MeTRH (3 μM), TRH + MeTRH, or TAL + MeTRH. The data are expressed as mean ± SEM performed in duplicate of two experiments.