Figure 3.
AZC treatment limits G1 cyclin activity. (A) Strains JVG961 (WT) and JVG1052 (cln1Δ.cln2Δ) were streaked on YPD plates containing AZC (7 mM) or no AZC and incubated at 30°C for 3 days. (B) Strains JVG961 (WT), JVG1022 (cdc28–1), JVG1023 (cdc28–9), and JVG1041 (cdc28–1N) were streaked on plates containing AZC (5 mM) or no AZC and were incubated at 23°C for 4 days. (C) Midlogarithmic cultures of JVG961 (S288C WT) transformants containing pCLN3–2 (pJO96) or a vector control (YCp50) growing in SD-URA at 23°C or 30°C were subjected to a temperature up-shift (TU: 23°C to 37°C) or were treated with AZC (10 mM, 30°C), respectively. The percentage of unbudded cells was determined for each culture as a function of time after treatment was initiated.