Figure 4. Overexpression of CCDC154 inhibits cell growth. (A) pEGFP-N1 was co-transfected to detect the transfection efficiency. Bar, 50 μm. (B−D) Transient transfection of CCDC154 inhibited human and mouse cell proliferation, as determined by the cell-counting assay and colony-formation assay. Mouse (mC) and human CCDC154 (hC) protein expression was detected by western blot using anti-myc antibody (upper panel). Vec, control vector. (E−G) Stable overexpression of CCDC154 suppressed human cell growth in cell-counting assay, MTT and colony formation assay. Vec is tranfected with control vector. hC-1# and hC-2# are two randomly selected human CCDC154 stable overexpression clones. Data shown are the mean results ± SD of a representative experiment performed in triplicate (n = 3), *p < 0.05, **p < 0.01 and ***p < 0.001.