Table 1. Sequences of oligonucleotides.

name

sequence

length1 [bp]

amount/ concentrations

UPL2 number

Electric Mobility Shift Assay (EMSA)
TBE3	ATTATTTCAAAACAAAGGTTTACAGAAA
TBE4	GAGTTACCCTCCTTTGATATTTTCTGTA
MUT3	ATTATTTCAACGCAGAGGTTTACAGAAA
MUT4	GAGTTACCCTCCTGTGCGATTTTCTGTA
c-Myc	CTAGCGCACCTTTGATTTCTGCACCTTTGATTTCTG

Chromatin immunoprecipitation (ChIP)
hTERT	ACTCGCGCTGCCCTTCTAGC ACGGTGTATCCCCAGTCTACGAAG	617	400 nM 400 nM
c-Myc	ACAGACGCCTCCCGCACGGG CCACACCGAGAACGCACTGC	451	400 nM 400 nM
GAPDH	TACTAGCGGTTTTACGGGCG TCGAACAGGAGGAGCAGAGAGCGA	165	400 nM 400 nM

RNA interference (RNAi)
β-catenin	CAGUUGUGGUUAAGCUCUUdTdT		60 nM
GFP	AAGCUACCUGUUCCAUGGCCAdTT		60 nM

RT-qPCR
β-catenin	AGCTGACCAGCTCTCTCTTCA CCAATATCAAGTCCAAGATCAGC	73	900 nM 900 nM	21
c-Myc	CACCAGCAGCGACTCTGA GATCCAGACTCTGACCTTTTGC	102	300 nM 300 nM	34
hTERT	CACGCGAAACCTTCCTC ACCACTGTCTTCCGCAAGTT	80	300 nM 900 nM	46
HPRT1	TGACCTTGATTTATTTTGCATACC CGAGCAAGACGTTCAGTCCT	102	900 nM 900 nM	73

¹ Length of amplicons resulting from gene specific PCRs.² UPL (universal probe library) given are the numbers of the probes of the UPL (Roche). In the EMSA probes TBE sequences are underlined.