Figure 1. In vitro and in vivo interaction between Pin1 and AR. Schematic representation of AR. (A) Potential Pin1 binding site targets on AR protein. (B) GST-Pin1 interaction with AR. A specific band is detected in the GST-PIN1 lane and no band is detected in GST control lane. (C) Pin1 interacts with AR in vivo. Cells were immunoprecipitated with anti-Pin1 antibody, analyzed by western blot with anti-AR antibody. The membrane was normalized with α-tubulin antibody. E, empty vector; OE, overexpression of Pin1; KD, Pin1 knockdown.