Figure 2. (A) Pin1 interacts with AR-NTD-DBD domain. The AR protein was split into two fragments and pulled down with GST-Pin1. Note no band is detected in GST control line. (B) The N-terminal domain NTD of AR was split into three fragments and pulled down with GST-Pin1. GST alone was used as the negative control. Pin1 interacts with NTD-A corresponding to the first 239 a.a. of AR. NTD, N-terminal domain; DBD, DNA binding domain; LDB, Ligand binding domain.