Figure 1. Overexpression of MIH1 is toxic to mck1∆ mutants in a CLB2-dependent fashion. (A) Overexpression of pGAL-GST-MIH1 (+) or vector alone (-) in wild type (WT), mck1∆, clb2∆ and clb2∆mck1∆ strains. (B) Overexpression of pGAL-CLB2-HA (+) or vector alone (-) in a WT or mck1∆ strain. Strains were grown to logarithmic phase, serially diluted onto the indicated media and grown for 4 d at 30°C.