Figure 2. Excision repair capacity of UV-induced photoproducts in mouse cells deficient in positive feedback loop of the core molecular clock. Exponentially growing, primary mouse embryonic fibroblast cells were irradiated with 5 J/m2 UVC, allowed to repair from 0–24 h, lysed and genomic DNA collected for immuno slot blot analysis. (A) An immuno slot blot assay was used to measure (6–4) PP repair at various time points post-irradiation with UV light. The image shows the (6–4) PP signal detected with an anti-(6–4) PP antibody and SYBR gold staining to show equal loading of total genomic DNA. (B) Quantitative analysis of the repair assay from (A) and experiments conducted under identical conditions. (C) An immuno slot blot assay was used to measure CPD repair at various time points, post-irradiation with UV light. The image shows the CPD signal detected with an anti-CPD antibody and SYBR gold staining to show equal loading of total genomic DNA. (D) Quantitative analysis of the repair assay from (C) and experiments conducted under identical conditions. The averages and standard deviations are from at least three independent experiments.