Figure 7. Effect of UV-induced apoptosis by reduced levels of circadian clock genes in mouse cells. siRNA transfected primary mouse skin fibroblast cells were irradiated with 0, 5, 10 or 25 J/m2 UVC, allowed to grow for 24 h and protein lysates were prepared for immunoblot analysis. (A) Protein levels were analyzed by immunoblotting 24 h after UV damage with the indicated antibodies. (B) Quantitative analysis of the immunoblot from three experiments. (C) Protein levels were analyzed by immunoblotting 24 h after UV damage with the indicated antibodies. (D) Quantitative analysis of the immunoblots. Averages and standard deviations are from at least three independent experiments. C-PARP and C-Caspase-3 stand for cleaved PARP and cleaved capsase-3, respectively.