Table 3.
Germline Phenotype at 18°C (%) | ||||
---|---|---|---|---|
Genotype | Wild Type | Mog2 | Pro3 | n1 |
rrf-14; glp-1(ar202gf); pL4440(RNAi)5 pL4440(RNAi)5 | 96 | 0 | 4 | 54 |
rrf-1; uaf-1(RNAi)6 | 49 | 51 | 0 | 49 |
rrf-1; glp-1(ar202gf); uaf-1(RNAi) | 32 | 15 | 53 | 34 |
Number of gonad arms
Masculinization of the germ line
Proximal proliferation. Gonads contain and over-proliferative tumor in the proximal end of the gonad.
rrf-1(pk1417)
pL4440 is an empty vector, used for control RNAi.
RNAi was performed by placing gravid hermaphrodites on RNAi plates and scoring the progeny, ensuring that progeny were not exposed to RNAi until after hatching. If uaf-1 RNAi was performed by placing L4 animals on the RNAi plate, this resulted in most of the progeny arresting as embryos, and a very small brood size.