| Initial Step | 30 seconds | 98 °C | (denaturation) |
| 18 to 25 cycles | 10 seconds | 98 °C | (denaturation) |
| 30 seconds | 65 °C | (annealing) | |
| 30 seconds | 72 °C | (extension) | |
| Final Step | 5 minutes | 72 °C | (final extension) |
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