Figure 4.

Flow cytometry analysis of anoxia-exposed zebrafish embryos. Embryos were collected and exposed to either a normoxic or an anoxic environment. Control embryos (normoxia) were at the shield stage of embryogenesis. Experimental embryos, arrested at the shield stage of embryogenesis, remained in the anoxic environment for 24 h and were immediately processed for flow cytometry analysis (anoxia). Regions 1 (R1) and 2 (R2) were defined to exclude debris and clumps of nuclei. DNA content histograms were determined by the fluorescent height histograms of gated events.