Table 3.
Effects of Different Concentrations of MP and FK506 on HBV DNA in HepG2.2.15 Cells (n=3)
| |
|
HBV DNA (log10 copies/mL) |
||
|---|---|---|---|---|
| Medication | Concentration (μg/L) | 24 h | 48 h | 72 h |
| MP | 0(1) | 7.0940±0.2389 | 6.7511±0.0577 | 6.3799±0.3973 |
| MP | 10(2) | 6.4632±0.2961 | 5.5023±0.2925 | 5.7821±1.1861 |
| MP | 50(3) | 5.3705±0.3239 | 5.1875±0.2211 | 4.6730±0.4476 |
| MP | 100(4) | 4.7231±0.0879 | 4.8842±0.1091 | 4.7865±0.0398 |
| MP | 250(5) | 4.5739±0.0735 | 4.4997±0.0248 | 4.1468±0.1016 |
| F | 69.053** | 73.358** | 30.366** | |
| r | −0.957 | −0.927 | −0.917 | |
| p | 0.000 | 0.000 | 0.000 | |
| FK506 | Control group(1) | 5.1126±0.1331 | 5.5691±0.1907 | 6.2491±0.1761 |
| FK506 | 50(2) | 4.6379±0.3692 | 4.7083±0.1963 | 6.1639±0.1354 |
| FK506 | 100(3) | 4.5368±0.2338 | 4.8246±0.3577 | 6.0457±0.1181 |
| FK506 | 500(4) | 4.4856±0.2583 | 5.2264±0.5135 | 6.0644±0.1817 |
| F | 3.579 | 3.987 | 1.110 | |
| r | −0.676 | −0.232 | −0.498 | |
| p | 0.016 | 0.469 | 0.099 | |
**
p<0.01.
HepG2.2.15 cells were treated with different concentrations of MP and FK506 as indicated to determine the effects on HBV DNA expression at different times. The results showed that HBV DNA expression decreased with increasing concentrations of MP. The differences were statistically significant between each concentration and the control group (0 μg/L). These results suggest that MP had a dose-dependent (but time-independent) inhibitory effect on HBV DNA in HepG2.2.15 cells. FK506 showed no significant effect on HBV DNA levels compared with the control group.
MP, methylprednisolone; FK506, tacrolimus; HBV, hepatitis B virus.