Table 6.

Amino Acid Mutation Sites in the S Gene and RT Region of the P Gene in Patients (n=14)

	Mutation in S region amino acid		Mutation in P region amino acid
Group	a-determinant	Outside a-determinant	LAM-resistant mutation	Outside LAM-resistant mutation
Before transplant	I126T(2), I126S(3), A128A(1), G130G(2), S136S(1), S143T(1), N146N(2)	S3N(13), G10A(4), Q30R(2), N40N, F41S L42R(3), A45A(3), T47A(3), T47K(3), T57I, N59S, P62L(3), S64C, I68T(6), F69L, G71G, F85C, L98V(5), Y100Y(4), L110F, I113S, T115T, T118T, K122K, A128A(2), Q129K(2), G145S(2), T148T, S155S(2), T160K, S171S(2), L175stop, L176L, L176Q, V177V, V184A(3), A185E(2), L186L, I195M(3), L209L, F212L(3), F212V(7), T538C(2)	rtL180M(2), rtM204V(2)	rtE11E(10), rtR18T(4), rtT38T, rtF49L, rtS50S(3), rtS53N, rtT54S(2), rtH55R(4), rtH55Q(2), rtN65Q, rtQ67Q, rtT70T, rtL72V, rtN76N(3), rtS78T, rtL93L, rtS106C(5), rtP109S(2), rtT118I, rtN121I, rtY124N, rtG127R, rtD131N, rtH133H, rtD134E(4), rtS137Q, rtN139H, rtL145M, rtF151Y, rtR153Q, rtK154Q, rtL155L(2), rtL157L, rtL164P(2), rtK168K, rtF183L, rtT184I, rtS185N(2), rtR192C, rtL220V(4), rtF221V(3), rtV217V
After transplant	T125M, T125T(2), I126T(3), I126S, G130G(6), T131N(4), S136S(3), S143T, D144D(3), N146N(4)	S3N(5), T4I, T5A, F8S, R24K, A45T, D45D, T47A(4), T47K, P49L, P56Q, T57I, N59S, S64C, I68T(3), R73R, I82I, F85C, Y100Y, L110F, L110R, T113T, T115T(2), T118T, K122K, T125T, I126T, G130G, G145S, T148T(2), S155S, T160K, F161Y, S167stop(2), S171S(2), L175stop(2), V177V(2), V184A(2), M198I(2), W199L, L203S, F212V(3), A288T	rtL180M	rtE11E(5), rtH12H, rtP13R, rtI16T, rtK32K, rtS53N, rtH55R(3), rtH55Q, rtP64P, rtN65Q(2), rtQ67Q, rtL72V, rtN76N(3), rtL81M, rtL93L, rtP109S, rtT118I, rtT118T, rtT118D, rtI122F, rtY124H, rtY124N(2), rtG127R, rtD131N, rtD134N(2), rtD134E, rtD134D(5), rtN139Q(3), rtN139H(3), rtL145M(4), rtL145M, rtF151Y, rtR153W, rtR153Q, rtR153W(2), rtK154Q, rtL155L(5), rtL157L(2), rtL164P, rtK168K, rtI169I, rtS176G(2), rtF183L(2), rtS185N, rtR192C(2), rtV207M(2), rtL220V(2)

Synonymous mutations in the S region before and after transplantation are shown in italics only. Underlined mutations are those in the RT region in the P gene overlapping the “a” determinant region of the S gene. Mutations that are both underlined and italicized are those in the RT region of the P region gene overlapping the S gene outside of the “a” determinant region. Numbers in brackets are frequencies of the mutations; no brackets indicates 1 frequency of mutation.