FIG. 2.

Effect of RM and RH on TNF-α-induced cell migration by inhibiting the MMP-9 activity in VSMC. Cells were treated with the indicated concentrations of RM and RH with the TNF-α (100 ng/mL) for 24 h. The supernatant of VSMC culture was prepared and used for gelatin zymography (black background). The pellets were used for Western blot (white background). β-actin was used as an internal control (A, B). A Matrigel migration assay was carried out with RM and RH with the TNF-α (100 ng/mL). After 12 h incubation, cells on the bottom side of the filter were fixed, stained, and counted (400×) (C). Data represent the mean±SEM of three independent experiments (n=3). *P<.05, **P<.01 when compared with the groups treated with LPS (100 ng/mL) alone. TNF, tumor necrosis factor; VSMC, vascular smooth muscle cell.