FIG. 3.

Effect of CA and CAR on LPS-induced MMP-9 secretion and MCP-1 expression in RAW 264.7 cells. Cells were pretreated with various concentrations of RM or RH for 1 h and induced by LPS (100 ng/mL) for an additional 23 h. The supernatants of cell culture were used for gelatin zymography (A, B). Total cell lysates were examined for MCP-1 protein expression (C, D) by Western blot. β-actin was used as an internal control. Data represent the mean±SEM of three independent experiments (n=3). **P<.01 when compared with the groups treated with LPS (100 ng/mL) alone. CAR, carnosol.