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Expression levels of MDR1 and MRP1 genes after 48 hours. A dose-dependent increase was observed for all ouabain doses combined with 2-DG. RT-PCR was used. GAPDH was used as the reference gene. The samples were studied in triplicate. The efficiency of the RT-PCR was 1. When compared with the control group, a ≥1.2-fold change was accepted as significant. MDR1, Multidrug resistant gene1; MRP-1, Multidrug resistance-associated protein1.
