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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Am J Transplant. 2011 Sep 22;12(1):69–80. doi: 10.1111/j.1600-6143.2011.03762.x

Figure 5. LFA-1 blockade attenuates CD8+ memory T cell effector responses.

Figure 5

(A) Intracellular cytokine staining of memory OT-I T cells harvested from C57BL/6 recipients on POD#7 after mOVA skin graft placement. Splenocytes were either left unstimulated or stimulated for four hours with OVA257-264 peptide (SIINFEKL). (B) LFA-1 blockade inhibits the ability of memory OT-I T cells to become dual-producers of TNF and IFN-gamma upon ex vivo restimulation. Results show (A) representative data or (B) summary of combined data from three independent experiments (5-6 mice/group). (C) CD107-based degranulation assay of memory OT-I T cells harvested from C57BL/6 recipients on POD#7 after mOVA skin graft placement. Splenocytes from these recipients were either left unstimulated (dashed line) or stimulated for four hours with SIINFEKL peptide (solid line). (D) Adjusted MFI of CD107a/b surface localization of memory OT-I T cells. Results show (C) representative data or (D) summary of combined data from two independent experiments (3-6 mice/group). (E and F) In vivo CTL of memory OT-I T cells in recipients of mOVA skin grafts to assess impact of different immunosuppression regimens on memory T cell cytotoxicity. Results show (E) representative data or (F) summary of combined data from five independent experiments (4-10 mice/group). All error bars represent the mean ± SEM.