Table 1.
Protection against B. malayi infective larvae in mice vaccinated with ALT-2pVAX using gene gun and intradermal delivery methods.
| Groups | Percentage protection | |
|---|---|---|
| In vitro ADCC assaya | In vivo micropore chamber assayb | |
| 5µg pVAX GG | 0.0 ± 0.0 | 0.0 ± 0.0 |
| 5µg BmALT-2 GG | 33.8 ± 5.3*** | 24.8 ± 3.4*** |
| 5µg BmALT-2 i.d. | 6.3 ± 8.8 | 5.5 ± 3.8 |
| 100µg BmALT-2 i.d. | 38.2 ± 2.6*** | 25.3 ± 6.2*** |
| 100µg pVAX i.d. | 0.0 ± 0.0 | 0.0 ± 0.0 |
a
ADCC assay was performed by incubating 50 µl of pooled mice sera (n = 5) samples with 0.5×105 normal peritoneal exudates cells and 10 B. malayi L3 at 37°C for 48 hrs. Sera from animals in each group were pooled and used in triplicates for the assay.
b
In vivo micropore chamber assay was performed by surgically implanting 20 B. malayi L3 into the peritoneal cavity of each mouse (N = 5). 48 hrs after implantation, chambers were removed and larval viability and death determined. All infective larvae recovered in the controls were alive.
***
Significant protection (P<0.001) as compared to control groups.