Figure 3. EVI1 overexpression is associated with cytokinesis defects. U2OS cells conditionally overexpressing EVI1-HA were monitored by time-lapse microscopy for 72 h following induction of transgene expression. A representative example of an EVI1-overexpressing cell undergoing mitosis is marked by arrowheads: after an apparently normal mitosis and karyokinesis, cytokinesis remains incomplete, resulting in a binuclear cell. Elapsed times after start of observation are given as hours:minutes.