Figure 7. EVI1-overexpressing cells harboring centrosome aberrations show low Ki-67 levels. (A) Parental U2OS cells, uninduced (-TET) and induced (+TET) U2OS cells conditionally overexpressing EVI1-HA were harvested 72 h after induction of transgene expression and immunostained for γ-tubulin (green) and Ki-67 (red). DNA was counterstained with DAPI (blue). Scale bars represent 10 µm. Insets show centrosomes at higher magnification. (B) U2OS cells conditionally overexpressing EVI1-HA were left untreated (-TET) or induced to express the transgene for 72 h (+TET). Parental U2OS cells were grown in parallel. Following immunostaining as in (A), percentages of cells with more than two2 centrosomes were quantified and given in relation to the Ki-67 staining intensity in 3 × 100 cells per data bar. ***, all indicated differences are highly significant (p < 0.0001).