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Comparison between the method described here for measuring lesions/base, and the RIN score, which is based on automated electrophoresis and which gives a result between 1 (totally degraded) and 10 (fully intact). Nine RNA samples were partially degraded by heating at various temperatures for 30 min and then analysed. The number of lesions/base shows a progressive increase as the temperature of heating increases whereas RIN shows a largely ‘all-or-nothing’ response.
