Figure 3.

In vivo kinetics of the elimination of aldehyde-induced DPCs. (A) Elimination of genomic DPCs after treatment with aldehydes. MRC5-SV cells were treated with the indicated aldehydes at LD₁₀ (Table 1) and incubated in fresh medium. DNA was isolated from cells after 0, 6 and 12 h of postincubation, and assayed for DPCs using the fluorometric format. (B) Elimination of aldehyde-induced genomic DPCs in NER-proficient MRC5-SV (WT) and NER-deficient XP12RO (XPA) cells. Experiments were performed with selected aldehydes (CAA and FA) as described for panel A. DPCs were detected using the western blotting format. Data points of panels A and B are means of three independent experiments with standard deviation.