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. 2012 Jul 5;40(18):9021–9035. doi: 10.1093/nar/gks617

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Nucleosome assembly of lamin B2 origin and its remodeling mediated by c-Myc recruitment. (A–D) HEK293 cells either starved or serum-stimulated were subjected to MNase-Southern assay. Ethidium bromide stained gel (left) and the corresponding hybridized blot (right) with probes LB2 ORI (A and B) and E-box (C and D) showing either the nucleosome array generated by partial MNase digestion (A and C) or mononucleosomal DNA obtained after complete MNase digestion (B and D). M-DNA marker lane. (E) MNase CHART-PCR analysis of HEK293 cells at different phases of cell cycle for both LB2 ORI and E-box regions. (F) HEK293 cells transfected with either vector control or Myc shRNA were subjected to MNase CHART-PCR analysis during G₁ phase. Data shown in (E and F) are mean ± SD of three independent experiments. The asterisk and number sign indicate statistically significant difference at P < 0.05 and P < 0.01, respectively.