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. 2012 Jul 5;40(18):9021–9035. doi: 10.1093/nar/gks617

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Methylation profiling of lamin B2 origin bearing E-box element. The genomic DNA isolated from G₀ and G₁ phases of HEK293 cells were processed for bisulphite (BS)-mediated base conversion as described in Materials and Methods, and DNA sequence information was obtained. The native DNA sequence (before BS treatment) corresponding to lamin B2 origin encompassing E-box (top: nucleotide 4179 to 4202, GenBank^TM accession number: M94363) was compared with sequence data of modified lamin B2 origin (after BS treatment). The unmethylated cytosines (Cs) got converted to Thymines (Ts) on BS treatment and marked as ‘+’, whereas the methylated Cs remained unchanged due to resistance to such conversion. The methylated CpG is denoted by upward triangle (blue). As cells progressed from G₀ to early G₁ phase_, the E-box region (green box flanked by dotted lines) underwent a demethylation event (downward triangle in red) confirming the demethylated status of this CpG.