Figure 1.

Scheme of the strategy to identify new miRNA targets. The number of detected target genes is indicated for each step of the analysis. After applying the conservation analysis, all genes with the same hit in the Arabidopsis proteome were considered as one target. Note that different genes with the same ID tag give only one hit, so that the total numbers of hits are reduced by this filter. Green squares refer to the target search using empirical filters: bins 5 and 6 include target genes selected by both evolutionary and empirical filters, while bins 2 and 3 have potential targets selected only by evolutionary filters.