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. 2012 Jul 5;40(18):8905–8916. doi: 10.1093/nar/gks647

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Small RNA sequencing in WT and ibm1 plants. (a) Scrolling-window analysis of small RNAs in ibm1-3 and WT. The reads produced by Illumina sequencing were mapped to the Arabidopsis genome, and the small RNA density distributions in 100-kb sliding windows with a 50-kb slide were counted along the chromosomes and are shown as the read number per bp. Bars with >10 reads/bp are not shown in this figure. (b) Comparison of the size distribution of small RNAs in WT and ibm1-3. The percentage of reads of a given length in all small RNA reads was calculated. (c) Scatterplot comparing the amounts of endogenous siRNAs generated from each locus between ibm1-3 and WT. The number of siRNA reads for each locus was counted and normalized to the scale of the libraries. RPM: reads per million.