Figure 8.
MiRNAs associate with different complexes in human milk. (A) P70 was prepared from fresh human milk and subjected to buoyant velocity centrifugation for 6 or 48 h. (B) Negative stain EM of particles in major CD81 peak void of measured miRNAs. Arrows: electron-poor globules only detectable in the peak CD81 fractions. (C) Graph of largest diameters of particles of CD81 peak fraction. (D) The abundance of indicated miRNAs were measured in each gradient fraction after 6 h or 48 h of centrifugation by TaqMan qRT–PCR. (E) Negative stain EM of particles in major miRNA containing fraction after 6 or 48 h of gradient centrifugation.
