Figure 5.

Spi-1 motif hits distribution inside Spi-1 peaks respects rule of engagment. (A) Distribution of the Spi-1 peak heights in the promoter + ImDown subregions (all peaks) or peaks in the promoter + ImDown subregions that contain (with CGI) or not (w/o CGI) CGIs and according to Spi-1 transcriptional activities. The red square indicates the mean, and the black line within the box plot indicates the median. The Kolmogorov–Smirnov test was used to statistically examine the differences in the distribution of the Spi-1 peak height compared with the peaks in the NoResp group of genes; P < 0.05 was considered statistically significant. Distribution of the distances between the pairs of Spi-1 motif hits in (B) head-to-tail orientation (the same strand) or (C) in head-to-head or tail-to-tail orientation (opposite strands) for activated genes. The X-axis presents the length of the spacers separating pairs of Spi-1 motif hits. The Y-axis shows the fraction of sequences with at least one pair of Spi-1 motif hits separated by the selected spacer (see the Supplementary Methods for details).